rat bone marrow mesenchymal stem cells mscs  (Procell Inc)

Journal: Materials Today Bio

Article Title: Targeted antibacterial and mesenchymal stem cell-modulatory hydrogel for periodontitis treatment

doi: 10.1016/j.mtbio.2026.103043

Figure Lengend Snippet: Schematic diagram of the ACP@Z@C hydrogel for periodontitis treatment. The BA-modified CC hydrogel for the delivery of CAPE-loading MOF, which accomplishes the targeted and controlled release of ZIF-8@CAPE in oral microenvironment. The released ZIF-8@CAPE interferes with multiple periodontitis-driven factors, including anti-bacteria, ROS-scavenging, and anti-inflammation. These potency transforms into periodontal tissue regeneration via rescuing the impaired osteogenic differentiation of MSCs.

Article Snippet: Rat bone marrow mesenchymal stem cells (MSCs) was purchased from Procell (Wuhan, China).

Techniques: Modification, Bacteria

Journal: Materials Today Bio

Article Title: Targeted antibacterial and mesenchymal stem cell-modulatory hydrogel for periodontitis treatment

doi: 10.1016/j.mtbio.2026.103043

Figure Lengend Snippet: Inhibiting inflammatory factor by adjusting mitochondrial dysfunction via SIRT1/p-AMPK/PGC-1α pathway. (A) Schematic illustration of the molecular mechanism by which Z@C regulates mitochondrial dysfunction and suppresses inflammatory factor production in MSCs. (B) Representative western blot bands and quantitative analysis of (C) SIRT1, (D) p-AMPK, (E) PGC-1α, (F) NLRP3, and (G) Pro-Caspase-1 protein expression. Data were presented as mean ± SD, n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. Expression levels of (H) SIRT1, (I) PGC-1α, (J) NLRP3, (K) IL-1β, (L) IL-6, and (M) TNF-α following Z@C treatment. Data were presented as mean ± SD, n = 5, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (N) Immunofluorescence staining of SIRT1 expression in MSCs following different groups and (O) quantitative analysis. Data were presented as mean ± SD, n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.

Article Snippet: Rat bone marrow mesenchymal stem cells (MSCs) was purchased from Procell (Wuhan, China).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining

Journal: RSC Advances

Article Title: Osteoinductive chemically crosslinked hydrogel enables hydroxyapatite formation, enhanced by release of dexamethasone, strontium, and zinc, and exhibits antimicrobial properties

doi: 10.1039/d5ra09258b

Figure Lengend Snippet: Effects of DEX and hydrogel crosslinker concentration on osteocalcin production by using rBM-MSCs. Asterisk signifies P ≤ 0.05. Error bars are standard deviation bars. n = 3 for each sample.

Article Snippet: Rat bone marrow mesenchymal stem cells (rBM-MSCs) were selected as cells that can form mineralized bone tissues, which are important in repairing bone fractures by differentiating into osteoblasts. rBM-MSCs were obtained from 28-day-old male Wistar rats (Charles River UK Ltd, Kent, UK), as previously described.

Techniques: Concentration Assay, Standard Deviation

Journal: Bioengineering & Translational Medicine

Article Title: Cell recognitive bioadhesive‐based osteogenic barrier coating with localized delivery of bone morphogenetic protein‐2 for accelerated guided bone regeneration

doi: 10.1002/btm2.10493

Figure Lengend Snippet: In vitro osteogenic behaviors of MSCs on the MAP‐RGD@BMP‐2‐coated surface. (a) Cell counts for the initial 3 days. (b) Western blot images of the protein expression of FAK, pY387‐FAK, BMPR‐IA, BMPR‐IB, and BMPR‐II in cells at 1 day after induction. (c) Relative quantitative levels of FAK phosphorylation and BMP‐2 receptor expression normalized to GAPDH. FAK activation was quantified through the relative band intensity of pY397‐FAK to FAK. (d) ALP activity at 4, 7, and 14 days after induction of differentiation. (e) Optical microscopic images and (f) calcium deposition levels of Alizarin Red S‐stained cells at 14 and 21 days after induction of differentiation. The data are presented as the mean ± SD with statistical significance (one‐way ANOVA with Tukey's post hoc test). p < 0.05 and p < 0.005 are noted as “a” and “c,” respectively. Statistical insignificance is noted as “ns.” BMP, morphogenetic protein‐2; Control, bare surface; MAP, mussel adhesive protein; MAP‐RGD, MAP‐RGD‐coated surface; MAP‐RGD@BMP‐2, MAP‐RGD@BMP‐2‐coated surface; RGD, Alg–Gly–Asp.

Article Snippet: Rat bone marrow mesenchymal stem cells (MSCs; ScienCell Research Laboratories, Carlsbad, CA, USA) were cultured and maintained in mesenchymal stem cell medium (ScienCell Research Laboratories) supplemented with 5% (v/v) FBS (ScienCell Research Laboratories), 1% (v/v) mesenchymal stem cell growth supplement (ScienCell Research Laboratories) and 1% (v/v) penicillin–streptomycin (ScienCell Research Laboratories) at 37°C in a humidified incubator containing 5% CO 2 and 95% air.

Techniques: In Vitro, Western Blot, Expressing, Phospho-proteomics, Activation Assay, Activity Assay, Staining, Control, Adhesive